Table 7 |
||||
| Gene-protein IHC/BISH (comparator)vs.single IHC/BISH (reference) agreement analyses | ||||
| Single IHC/BISH assay | ||||
| Gene-protein IHC/BISH | Positive | Negative | Total | |
| Reader A | Positive | 50 | 2 | 52 |
| Negative | 1 | 112 | 113 | |
| Total | 51 | 114 | 165 | |
| PPA: n/N (%) (95% CI) | 50/51 (98.0) (89.7–99.7) | |||
| NPA: n/N (%) (95% CI) | 112/114 (98.2) (93.8–99.5) | |||
| OPA: n/N (%) (95% CI) | 162/165 (98.2) (94.8–99.4) | |||
| Kappa coefficient (95% CI) | 0.96 (0.91–1.00) | |||
| Reader B | Positive | 50 | 2 | 52 |
| Negative | 3 | 117 | 120 | |
| Total | 53 | 119 | 172 | |
| PPA: n/N (%) (95% CI) | 50/53 (94.3) (84.6–98.1) | |||
| NPA: n/N (%) (95% CI) | 117/119 (98.3) (94.1–99.5) | |||
| OPA: n/N (%) (95% CI) | 167/172 (97.1) (93.4–98.8) | |||
| Kappa coefficient (95% CI) | 0.93 (0.87–0.99) | |||
| Reader C | Positive | 52 | 3 | 55 |
| Negative | 2 | 116 | 118 | |
| Total | 54 | 119 | 173 | |
| PPA: n/N (%) (95% CI) | 52/54 (96.3) (87.5–99.0) | |||
| NPA: n/N (%) (95% CI) | 116/119 (97.5) (92.8–99.1) | |||
| OPA: n/N (%) (95% CI) | 168/173 (97.1) (93.4–98.8) | |||
| Kappa coefficient (95% CI) | 0.93 (0.88–0.99) | |||
IHC: immunohistochemistry; BISH: brightfield in situ hybridization; PPA: positive percent agreement; NPA: negative percent agreement; OPA: overall percent agreement.
Note: IHC/BISH status: positive = IHC is positive or BISH is amplified; negative = IHC is negative and BISH is non-amplified.
Nitta et al. Diagnostic Pathology 2012 7:60 doi:10.1186/1746-1596-7-60
