Table 7

Gene-protein IHC/BISH (comparator)vs.single IHC/BISH (reference) agreement analyses
Single IHC/BISH assay
Gene-protein IHC/BISH Positive Negative Total
Reader A Positive 50 2 52
Negative 1 112 113
Total 51 114 165
PPA: n/N (%) (95% CI) 50/51 (98.0) (89.7–99.7)
NPA: n/N (%) (95% CI) 112/114 (98.2) (93.8–99.5)
OPA: n/N (%) (95% CI) 162/165 (98.2) (94.8–99.4)
Kappa coefficient (95% CI) 0.96 (0.91–1.00)
Reader B Positive 50 2 52
Negative 3 117 120
Total 53 119 172
PPA: n/N (%) (95% CI) 50/53 (94.3) (84.6–98.1)
NPA: n/N (%) (95% CI) 117/119 (98.3) (94.1–99.5)
OPA: n/N (%) (95% CI) 167/172 (97.1) (93.4–98.8)
Kappa coefficient (95% CI) 0.93 (0.87–0.99)
Reader C Positive 52 3 55
Negative 2 116 118
Total 54 119 173
PPA: n/N (%) (95% CI) 52/54 (96.3) (87.5–99.0)
NPA: n/N (%) (95% CI) 116/119 (97.5) (92.8–99.1)
OPA: n/N (%) (95% CI) 168/173 (97.1) (93.4–98.8)
Kappa coefficient (95% CI) 0.93 (0.88–0.99)

IHC: immunohistochemistry; BISH: brightfield in situ hybridization; PPA: positive percent agreement; NPA: negative percent agreement; OPA: overall percent agreement.

Note: IHC/BISH status: positive = IHC is positive or BISH is amplified; negative = IHC is negative and BISH is non-amplified.

Nitta et al.

Nitta et al. Diagnostic Pathology 2012 7:60   doi:10.1186/1746-1596-7-60

Open Data