Table 6

Gene-protein BISH (comparator)vs.single BISH (reference) agreement analyses
Single BISH
Gene-protein BISH Amplified Non-amplified Total
Reader A Amplified 43 2 45
Non-amplified 2 118 120
Total 45 120 165
PPA: n/N (%) (95% CI) 43/45 (95.6) (85.2–98.8)
NPA: n/N (%) (95% CI) 118/120 (98.3) (94.1–99.5)
OPA: n/N (%) (95% CI) 161/165 (97.6) (93.9–99.1)
Kappa coefficient (95% CI) 0.94 (0.88–1.00)
Reader B Amplified 47 1 48
Non-amplified 3 121 124
Total 50 122 172
PPA: n/N (%) (95% CI) 47/50 (94.0) (83.8–97.9)
NPA: n/N (%) (95% CI) 121/122 (99.2) (95.5–99.9)
OPA: n/N (%) (95% CI) 168/172 (97.7) (94.2–99.1)
Kappa coefficient (95% CI) 0.94 (0.89–1.00)
Reader C Amplified 41 5 46
Non-amplified 2 125 127
Total 43 130 173
PPA: n/N (%) (95% CI) 41/43 (95.3) (84.5–98.7)
NPA: n/N (%) (95% CI) 125/130 (96.2) (91.3–98.3)
OPA: n/N (%) (95% CI) 166/173 (96.0) (91.9–98.0)
Kappa coefficient (95% CI) 0.89 (0.82–0.97)

BISH: brightfield in situ hybridization; PPA: positive percent agreement; NPA: negative percent agreement; OPA: overall percent agreement.

Nitta et al.

Nitta et al. Diagnostic Pathology 2012 7:60   doi:10.1186/1746-1596-7-60

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