Table 5

Gene-protein IHC (comparator)vs.single IHC (reference) agreement analyses
Single IHC
Gene-protein IHC Positive Negative Total
Reader A Positive 50 2 52
Negative 0 131 131
Total 50 133 183
PPA: n/N (%) (95% CI) 50/50 (100.0) (92.9–100.0)
NPA: n/N (%) (95% CI) 131/133 (98.5) (94.7–99.6)
OPA: n/N (%) (95% CI) 181/183 (98.9) (96.1–99.7)
Kappa coefficient (95% CI) 0.97 (0.94–1.00)
Reader B Positive 47 3 50
Negative 1 132 133
Total 48 135 183
PPA: n/N (%) (95% CI) 47/48 (97.9) (89.1–99.6)
NPA: n/N (%) (95% CI) 132/135 (97.8) (93.7–99.2)
OPA: n/N (%) (95% CI) 179/183 (97.8) (94.5–99.1)
Kappa coefficient (95% CI) 0.94 (0.89–1.00)
Reader C Positive 54 1 55
Negative 0 128 128
Total 54 129 183
PPA: n/N (%) (95% CI) 54/54 (100.0) (93.4–100.0)
NPA: n/N (%) (95% CI) 128/129 (99.2) (95.7–99.9)
OPA: n/N (%) (95% CI) 182/183 (99.5) (97.0–99.9)
Kappa coefficient (95% CI) 0.99 (0.96–1.00)

IHC: immunohistochemistry; PPA: positive percent agreement; NPA: negative percent agreement; OPA: overall percent agreement.

Nitta et al.

Nitta et al. Diagnostic Pathology 2012 7:60   doi:10.1186/1746-1596-7-60

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