Table 2 |
|
| Targeted Mutations and Pyrosequencing dispensing order for EGFR, KRAS and BRAF mutations | |
| Targeted Mutations | Pyrosequencing Dispensing Orders |
| EGFR exon 18, codon 719: | CATGTCACTCGTG |
| EGFR exon 19 deletions | CTATCACTGTCAGCTCGATCGTCATCGTCACGC |
|
EGFR exon 20, Codon 768 and insertions |
GCAGTACGTGTCGTGTACGTGACCACACTG |
| EGFR exon 20, Codon 790 | GATTCATCTG |
| EGFR exon 21 | ACGTGTCACATGTC |
| KRAS codon 12 & 13 | ACTGTACGTGATCGTAGCAAGAG |
| KRAS codon 61 (reverse sequencing) | GCTCGATACGACCT |
| BRAF codon 600 (reverse sequencing) | TCGTATCTGTAG |
Shen and Qin Diagnostic Pathology 2012 7:56 doi:10.1186/1746-1596-7-56
