Table 1 |
|
| Targeted Mutations and Sequences for EGFR, KRAS and BRAF mutations | |
| Targeted Mutations | Targeted Sequences |
| EGFR exon 18, codon 719 | DSCTCCGGTGC |
| EGFR exon 19 deletions | TATCAA[GGAATTAAGAGAAGC]AACATCTCCGAAAGCCA |
| EGFR exon 20, Codon 768 | CAGCGTGGACAACCCCCACG |
| EGFR exon 20, Codon 790 | ATCAYG |
| EGFR exon 21 | CKGGCCAAACDGCTGGGT |
| KRAS codon 12 & 13 | NNTGRCGTAGGC |
| KRAS codon 61 (reverse sequencing) | CTCDTGACCTG |
| BRAF codon 600 (reverse sequencing) | CWCTGTAG |
Note: “D” could be A, T, U, or G (but not C); “S” could be C or G; “Y” could be C, T or U; “N” could any base; “R” could be A or G; “W”: T, U, or A. “[GGAATTAAGAGAAGC]” indicates deletion of the sequence.
Shen and Qin Diagnostic Pathology 2012 7:56 doi:10.1186/1746-1596-7-56
