Open Access Research

Evaluation of inadequate, indeterminate, false-negative and false-positive cases in cytological examination for breast cancer according to histological type

Rin Yamaguchi1237*, Shin-ichi Tsuchiya4, Takashi Koshikawa5, Toshiro Yokoyama3, Kuniko Mibuchi3, Yasuhide Nonaka3, Sonoe Ito3, Hidejiro Higuchi3, Mariko Nagao3, Koichi Higaki3, Jiro Watanabe3, Masayoshi Kage6 and Hirohisa Yano2

Author Affiliations

1 Department of Pathology, Kurume University Medical Center, Kurume, Japan

2 Department of Pathology, Kurume University School of Medicine, Kurume, Japan

3 Working Team for the Accuracy of Breast Cytology, Southern Fukuoka Prefecture, Fukuoka, Japan

4 Department of Diagnostic Pathology, Nippon Medical School Hospital, Tokyo, Japan

5 Department of Pathology, Aichi Prefectural University, School of Nursing & Health, Aichi, Japan

6 Department of Diagnostic Pathology, Kurume University Hospital, Kurume, Japan

7 Department of Pathology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan

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Diagnostic Pathology 2012, 7:53  doi:10.1186/1746-1596-7-53

Published: 18 May 2012

Abstract

Background

We previously investigated the current status of breast cytology cancer screening at seven institutes in our area of southern Fukuoka Prefecture, and found some differences in diagnostic accuracy among the institutions. In the present study, we evaluated the cases involved and noted possible reasons for their original cytological classification as inadequate, indeterminate, false-negative and false-positive according to histological type.

Methods

We evaluated the histological findings in 5693 individuals who underwent cytological examination for breast cancer (including inadequate, indeterminate, false-negative and false-positive cases), to determine the most common histological types and/or features in these settings and the usefulness/limitations of cytological examination for the diagnosis of breast cancer.

Results

Among 1152 cytologically inadequate cases, histology revealed that 75/173 (43.6%) cases were benign, including mastopathy (fibrocystic disease) in 38.6%, fibroadenoma in 24.0% and papilloma in 5.3%. Ninety-five of 173 (54.9%) cases were histologically malignant, with scirrhous growing type, invasive ductal carcinoma (SIDC) being significantly more frequent (49.5%) than papillotubular growing type (Papi-tub) (P < 0.0001), solid-tubular growing type (P = 0.0001) and ductal carcinoma in situ (DCIS) (P = 0.0001). Among 458 indeterminate cases, 54/139 (38.8%) were histologically benign (mastopathy, 30.0%; fibroadenoma, 27.8%; papilloma, 26.0%) and 73/139 (52.5%) were malignant, with SIDC being the most frequent malignant tumor (37.0%). Among 52 false-negative cases, SIDC was significantly more frequent (42.3%) than DCIS (P = 0.0049) and Papi-tub (P = 0.001). There were three false-positive cases, with one each of fibroadenoma, epidermal cyst and papilloma.

Conclusions

The inadequate, indeterminate, false-negative and false-positive cases showed similar histological types, notably SIDC for malignant tumors, and mastopathy, fibroadenoma and papilloma for benign cases. We need to pay particular attention to the collection and assessment of aspirates for these histological types of breast disease. In particular, several inadequate, indeterminate and false-negative cases with samples collected by aspiration were diagnosed as SIDC. These findings should encourage the use of needle biopsy rather than aspiration when this histological type is identified on imaging. Namely, good communication between clinicians and pathological staff, and triple assessment (i.e., clinical, pathological and radiological assessment), are important for accurate diagnosis of aspiration samples.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7349809170055423 webcite

Keywords:
Breast cytology; False-negative; False-positive; Indeterminate; Inadequate