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Open Access Research

Expression of GSK-3β in renal allograft tissue and its significance in pathogenesis of chronic allograft dysfunction

Qiang Yan1, Baoyao Wang2, Weiguo Sui1, Guimian Zou1, Huaizhou Chen1, Shenping Xie1 and Hequn Zou3*

Author affiliations

1 Kidney Tranplantation and Dialysis Center of PLA NO.181 Hospital, Clinical College of Southern Medical University, Guilin 524001, Guangxi, P.R.China

2 Southern Medical University, Guangzhou 510630, Guangdong, P.R.China

3 Institute of Urology and Nephrology, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China

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Citation and License

Diagnostic Pathology 2012, 7:5  doi:10.1186/1746-1596-7-5

Published: 13 January 2012

Abstract

Objective

To explore the expression of Glycogen synthase kinase 3 beta (GSK-3β) in renal allograft tissue and its significance in the pathogenesis of chronic allograft dysfunction.

Methods

Renal allograft biopsy was performed in all of the renal allograft recipients with proteinuria or increased serum creatinine level who came into our hospital from January 2007 to December 2009. Among them 28 cases was diagnosed as chronic allograft dysfunction based on pahtological observation, including 21 males with a mean age of 45 ± 10 years old and 7 females with a mean age of 42 ± 9 years old. The time from kidney transplantation to biopsy were 1-9 (3.5) years. Their serum creatinine level were 206 ± 122 umol/L. Immunohistochemical assay and computer-assisted genuine color image analysis system (imagepro-plus 6.0) were used to detect the expression of GSK-3β in the renal allografts of 28 cases of recipients with chronic allograft dysfunction. Mean area and mean integrated optical density of GSK-3β expression were calculated. The relationship between expression level of GSK-3β and either the grade of inflammatory cell infiltration or interstitial fibrosis/tubular atrophy in renal allograft was analyzed. Five specimens of healthy renal tissue were used as controls.

Results

The expression level of the GSK-3β was significantly increased in the renal allograft tissue of recipients with chronic allograft dysfunction, compared to normal renal tissues, and GSK-3β expression became stronger along with the increasing of the grade of either inflammatory cell infiltration or interstitial fibrosis/tubular atrophy in renal allograft tissue.

Conclusion

There might be a positive correlation between either inflammatory cell infiltration or interstitial fibrosis/tubular atrophy and high GSK-3β expression in renal allograft tissue.

Virtual slides

The virtual slide(s) for this article can be found here:

http://www.diagnosticpathology.diagnomx.eu/vs/9924478946162998 webcite.

Keywords:
kidney transplantation; GSK-3β; inflammatory cell infiltration