Table 1

Panel of immunohistochemical stains

Immunohistochemical stains

Clone

Sources

Dilution

Results


Pan cytokeratin

AE1/AE3

Neomarkers

1:100

Neg.

Keratin 8/18

5D3

Neomarkers

1:200

Neg.

Keratin 19

RCK108

Neomarkers

1:200

Neg.

Renal Cell Carcinoma Marker(gp200)

PN-15

Neomarkers

1:100

Neg.

Neuron-specific enolase (NSE)

NSE-P1

Neomarkers

1:1500

Pos.

Epithelial membrane antigen (EMA)

E29

Neomarkers

1:500

Pos. (focal)

CD10

56 C6

Neomarkers

1:50

Pos. (focal)

Ki-67

SP6

Neomarkers

1:500

Pos. (1%)

Vimentin

SP20

Neomarkers

1:300

Pos.

S100 protein

Polyclonal antiserum

Dako

1:6000

Pos.

a-inhibin

R1

Dako

1:1250

Pos.

Epidermal growth factor receptor (EGFR)

EP38Y

Neomarkers

1:100

Pos.

calretinin

SP13

Neomarkers

1:100

Neg.

Melanoma Marker

HMB45

Neomarkers

1:50

Neg.

Melan-A

A103

Neomarkers

1:100

Neg.

Chromogranin A

SP12

Neomarkers

1:200

Neg.

Desmin

Rabbit Polyclonal Antibody

Neomarkers

1:200

Neg.

Actin

HHF35

Neomarkers

1:100

Neg.

Myoglobin

Rabbit Polyclonal Antibody

Neomarkers

1:800

Neg.

CD68

PG-M1

Neomarkers

1:100

Neg.

CD34

QBEnd/10

Neomarkers

1:80

Neg.


Yin et al. Diagnostic Pathology 2012 7:39   doi:10.1186/1746-1596-7-39

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