Open Access Research

Validation of diagnostic accuracy using digital slides in routine histopathology

László Fónyad1*, Tibor Krenács1, Péter Nagy1, Attila Zalatnai1, Judit Csomor1, Zoltán Sápi1, Judit Pápay1, Júlia Schönléber1, Csaba Diczházi1 and Béla Molnár2

Author Affiliations

1 First Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

2 Second Department of Internal Medicine, Semmelweis University, Budapest, Hungary

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Diagnostic Pathology 2012, 7:35  doi:10.1186/1746-1596-7-35

Published: 31 March 2012

Additional files

Additional file 1:

Figure S1. User interface of the Mirax DataBase software. Field 1. shows the Available Projects, where the cases submitted to the specific pathologist is listed, allowing the pathologist to organize the cases by setting up four different statuses, such as: New, Examined, Diagnosed or Reopened. First every DS were presented in a separate List of New Slides. (Field 2.) Later, any newly requested and uploaded slide (recuts, special stains, IHC) appeared here for limiting the chance of not using any single slide before signing out. In the Project Database and Preview (Field 3.) the Scanned Slides and their metadata (slide properties, attachments, direct links to slide annotations) can be seen. Field 4., as a slide-box, shows the thumbnail view of the slides of the active case. In. Field 5. clinical data and the gross description of the specimen is given in addition with results of the prehistological examinations such as cytology. Results of special diagnostic procedures, such as flow cytometry, molecular diagnostics, electronmicroscopy etc., were also edited here.

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Additional file 2:

Figure S2. Incomplete scan. (Case 6., H&E, digital diagnose: fibrosis mammae; consensus diagnose: sine morbo)

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Additional file 3:

Figure S3. Scan is out of focus. (Case 45., H&E, digital diagnose: chronic bronchitis, adenocarcinoma?; consensus diagnose: chronic bronchitis)

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Additional file 4:

Figure S4. Scan is out of focus, poor color fidelity. (Case 226., Giemsa, digital diagnose: moderate chronic, active, aspecific gastritis; consensus diagnose: severe chronic, active, HP-associated gastritis)

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Additional file 5:

Table S1. Incoherent cases. According to the type of incoherency the table highlights the origin of the samples, pathologist, and original and consensus diagnosis as well as data about diagnostic confidence from the Clinical Research Form. Table S2. Influance of sample origin. The table shows the collected data according to sample origin, highlighting organs where the organ specific incoherency ratio is below 8.82% (ratio of all reassesed cases). Table S3. Pathologist competence. The table shows the collected data on how the diagnostic confidence, reasons of uncertainty and ratio of the diagnostic errors changed after excluding non-field specific cases from each pathologists' record.

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