The examples of immunohistochemistry and digital analysis output images. Immunohistochemistry and corresponding digital analysis outputs of SATB1 (a and b), HIF-1α (c and d), and BCL2 (e and f). The Nuclear algorithm (b and d) marks the positive cells with color mask according staining intensity (0 - blue, 1+ - yellow, 2+ - orange, 3+ - red). The Membrane algorithm (f) marks the positive cells with complete membranous staining with red outline.
Laurinavicius et al. Diagnostic Pathology 2012 7:27 doi:10.1186/1746-1596-7-27