Figure 1.

The examples of immunohistochemistry and digital analysis output images. Immunohistochemistry and corresponding digital analysis outputs of SATB1 (a and b), HIF-1α (c and d), and BCL2 (e and f). The Nuclear algorithm (b and d) marks the positive cells with color mask according staining intensity (0 - blue, 1+ - yellow, 2+ - orange, 3+ - red). The Membrane algorithm (f) marks the positive cells with complete membranous staining with red outline.