Figure 4.

False-color imaging. Immunohistochemical detection of pHp in HOPE-fixed, paraffin embedded human lung tissues. Image-acquisition was performed using a Leica DM-LB2-microscope with 40x PL-Fluotar optics and a Leica DFC 320 camera with Leica software (Leica Microsystems, Germany). White balance, and contrast were adjusted using FixFoto (Koopmann, Germany). A: Same as Figure‚ÄČ1B (400x), B: the same specimen with color inversion; pHp now in blue color (400X), C and D: Two other examples of color-inverted photomicrographs showing a film of pHp in the intended position of the surfactant in human alveoli (400x). Examples of more than 10 experiments.

Abdullah and Goldmann Diagnostic Pathology 2012 7:158   doi:10.1186/1746-1596-7-158
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