(a) Giemsa stained section of Hep2-treated cells showing sampling of the color to be thresholded. (b) Thresholding of the color and watershed of 8-bit image. (c) Analyzing particles after excluding edges. (d) Drawing outlines and automatic numbering of the desired objects.
Helmy and Abdel Azim Diagnostic Pathology 2012 7:15 doi:10.1186/1746-1596-7-15