Double-color probe set for the detection of the hTERC gain and immunohistological detection for hTERT in cervical dysplasia.A. interphase FISH shows a normal signal pattern containing two copies each of the hTERC ( red) and CEP3 (green) (X630). B. interphase FISH shows increased number of signals for hTERC (red) compared to CEP3(green) (X630) C. interphase FISH shows gain of both hTERC (red) and CEP3 (green) gains in the larger cells (X630). D. Weakly hTERT positive cells are found in the suprabasal cell layers. (IHC for hTERT, original magnification× 400) E. A high-grade CIN3 lesion, where hTERT positive cells are found throughout the full thickness of the epithelium. (IHC for hTERT, original magnification×400) F. An invasive squamous cell carcinoma with strong hTERT expression. The staining intensity varies from weak to intense dark brown, indicating that the expression levels of hTERT vary from cell to cell. (IHC for hTERT, original magnification× 400).
Liu et al. Diagnostic Pathology 2012 7:147 doi:10.1186/1746-1596-7-147