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Open Access Research

Ezrin overexpression predicts the poor prognosis of gastric adenocarcinoma

Jingchun Jin14, Tiefeng Jin12, Meiling Quan12, Yingshi Piao23 and Zhenhua Lin12*

Author Affiliations

1 Department of Pathology, Yanbian University Medical College, Yanji-City, 133002, Jilin-Prov., P.R. China

2 Cancer Research Center, Yanbian University, Yanji-City, 133002, Jilin-Prov., P.R. China

3 Department of Pathophysiology, Yanbian University Medical College, Yanji-City, 133002, Jilin-Prov., P.R. China

4 Department of Internal Medicine, Yanbian University Affiliated Hospital, Yanji-City, 133000, Jilin-Prov., P.R. China

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Diagnostic Pathology 2012, 7:135  doi:10.1186/1746-1596-7-135

Published: 5 October 2012

Abstract

Background

Ezrin is a cytoskeletal protein that is involved in tumor growth and invasion. It has been suggested that Ezrin expression plays an important role in tumor metastasis. This study is aimed to investigate the clinicopathological significance of Ezrin overexpression in gastric adenocarcinomas.

Methods

Ezrin protein expression was examined by immunohistochemistry in 26 normal gastric mucosa, 32 dysplasia, and 277 gastric adenocarcinomas. The relationship between Ezrin expression and the clinicopathological features of gastric cancers was analyzed. In addition, a gastric cancer cell line, MKN-1, was also used for immunofluorescence staining to evaluate the distribution of Ezrin protein.

Results

Ezrin protein located in the cytoplasm and/or membrane in the migrating gastric cancer cells, and it mainly concentrated at the protrusion site; however, only cytoplasmic distribution was observed in the non-migrating cancer cells by immunofluorescence staining. The positive rate of Ezrin protein expression was significantly higher in gastric adenocarcinoma and dysplasia compared with that in the normal gastric mucosa. Moreover, expression frequency of Ezrin protein increased significantly in lymph node metastasis and late clinical stages. Consistently, strong expression of Ezrin was significantly correlated with poor prognosis of gastric cancer.

Conclusion

The detection of Ezrin expression can be used as the marker for early diagnosis and prognosis of gastric adenocarcinoma.

Virtual Slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2303598677653946

Keywords:
Gastric adenocarcinoma; Ezrin; Tissue microarray