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Molecular correlates and prognostic significance of SATB1 expression in colorectal cancer

Björn Nodin1, Henrik Johannesson2, Sakarias Wangefjord1*, Darran P O’Connor3, Kajsa Ericson Lindquist1, Mathias Uhlén45, Karin Jirström1 and Jakob Eberhard6

Author Affiliations

1 Department of Clinical Sciences, Pathology, Lund University, Skåne University Hospital, Lund, SE, 221 85, Sweden

2 Atlas Antibodies AB, AlbaNova University Center, Stockholm, SE, 106 91, Sweden

3 UCD School of Biomolecular and Biomedical Science, UCD Conway Institute, University College Dublin, Dublin 4, Belfield, Ireland

4 Science for Life Laboratory, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden

5 School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden

6 Department of Clinical Sciences, Oncology, Lund University, Skåne University Hospital, Lund, SE, 221 85, Sweden

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Diagnostic Pathology 2012, 7:115  doi:10.1186/1746-1596-7-115

Published: 30 August 2012

Additional files

Additional file 1:

Sample immunohistochemical images of beta-catenin grades. Sample images of beta-catenin staining representing (A) normal colorectal epithelial cells with intact membranous beta-catenin expression, and colorectal cancers with (B) intact membranous expression (grade 1), (C) positive cytoplasmic expression (grade 2), and (D) positive nuclear and cytoplasmic expression (grade 3).

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Additional file 2:

Validation of the anti-SATB1 antibody. The specificity of the anti-SATB1 antibody was analysed using (A) ELISA and (B) Western blot against purified recombinant SATB1 and SATB2 proteins. An anti-SATB2 antibody was included as a control in the western blot experiment, which shows that both antibodies are specific for their respective proteins.

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Additional file 3:

Distribution of SATB1 staining in the full cohort. Distribution of all nuclear scores (fraction x intensity) of SATB1 expression in the full cohort. Percentage is shown on the y-axis and absolute numbers above the bars.

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