Table 2

Primers used for PCR
Gene Sequence of primers Size of PCR(bp) Denaturation temperature (°C) Cycles
ASK1 sense 5’-TTCACACAAAACGGATGTAACATT-3’ 198 56 30
antisense 5’-CCTAAACAGTTATGGTCACATTTTGG-3’
Claudin-6 sense 5’-TTCATCGGCAACAGCATCGT-3’ 345 58 35
antisense 5’-GGTTATAGAAGTCCCGGATGA-3’
GAPDH sense 5’-TGTTGCCATCAATGACCCCTT-3’ 202 56 25
antisense 5’-CTCCACGACGTACTCAGCG-3’

Guo et al.

Guo et al. Diagnostic Pathology 2012 7:111   doi:10.1186/1746-1596-7-111

Open Data