Table 2 |
|||||
| Primers used for PCR | |||||
| Gene | Sequence of primers | Size of PCR(bp) | Denaturation temperature (°C) | Cycles | |
| ASK1 | sense | 5’-TTCACACAAAACGGATGTAACATT-3’ | 198 | 56 | 30 |
| antisense | 5’-CCTAAACAGTTATGGTCACATTTTGG-3’ | ||||
| Claudin-6 | sense | 5’-TTCATCGGCAACAGCATCGT-3’ | 345 | 58 | 35 |
| antisense | 5’-GGTTATAGAAGTCCCGGATGA-3’ | ||||
| GAPDH | sense | 5’-TGTTGCCATCAATGACCCCTT-3’ | 202 | 56 | 25 |
| antisense | 5’-CTCCACGACGTACTCAGCG-3’ | ||||
Guo et al. Diagnostic Pathology 2012 7:111 doi:10.1186/1746-1596-7-111
