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Open Access Research

Laminin-5 is a biomarker of invasiveness in cervical adenocarcinoma

Johji Imura1*, Yoshiaki Uchida2, Kazuhiro Nomoto1, Kazuhito Ichikawa3, Shigeki Tomita3, Tatsuo Iijima2 and Takahiro Fujimori3

Author Affiliations

1 Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama City, Toyama, 930-0194, Japan

2 Department of Pathology, Ibaraki Prefectural Central Hospital, 6528 Koibuchi, Kasama, Ibaraki, 309-1793, Japan

3 Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi, 321-0293, Japan

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Diagnostic Pathology 2012, 7:105  doi:10.1186/1746-1596-7-105

Published: 17 August 2012

Abstract

Background

Glandular lesions are often problematic for diagnostic cervical pathology. The survival of patients with adenocarcinoma is significantly poorer than that of patient with squamous cell carcinoma. One reason for this increased risk is the aggressive invasiveness of adenocarcinoma. Therefore additional biomarkers, to supplement morphological diagnosis of adenocarcinoma, are necessary. We have assessed the diagnostic utility of Laminin-5 (Laminin γ2 chain): Lam-5 in the diagnosis of the invasiveness of cervical adenocarcinoma and related glandular lesions.

Methods

Lam-5 immunohistochemistry was performed on archival specimens from 8 patients with uterine leiomyoma as a negative control group, 6 patients with endocervical gland hyperplasia, 6 patients with adenocarcinoma in situ, 6 patients with microinvasive adenocarcinoma and 24 patients with invasive adenocarcinoma.

Results

The expression of Lam-5 was not detected in normal mucosa, but was seen along the basement membrane in endocervical gland hyperplasia and adenocarcinoma in situ and was observed in the cytoplasm of tumor cells in microinvasive and invasive adenocarcinoma.

Conclusion

We conclude that Lam-5 is a useful biomarker in the evaluation of invasiveness in cervical adenocarcinoma.

Virtual slides

The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7316562925827381

Keywords:
Cervical adenocarcinoma; Invasiveness; Laminin-5; Immunohistochemical analysis