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This article is part of the supplement: Proceedings of the 10th European Congress on Telepathology and 4th International Congress on Virtual Microscopy

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Localization of Eosinophilic Esophagitis from H&E stained images using multispectral imaging

Pinky A Bautista* and Yukako Yagi

Author Affiliations

Department of Pathology, Massachusetts General Hospital, Harvard Medical School Boston, MA,USA

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Diagnostic Pathology 2011, 6(Suppl 1):S2  doi:10.1186/1746-1596-6-S1-S2

Published: 30 March 2011


This study is an initial investigation on the capability of multispectral imaging to capture subtle spectral information that would enable the automatic delineation between the eosinophilic esophagitis and other eosin stained tissue components, especially the RBCs. In the method, a principal component analysis (PCA) was performed on the spectral transmittance samples of the different tissue components, excluding however the transmittance samples of the eosinophilic esophagitis. From the average spectral error configuration of the eosinophilic esophagitis transmittance samples, i.e. the difference between the actual transmittance and the estimated transmittance using m PC vectors, we indentified two spectral bands by which we can localize the eosinophils. Initial results show the possibility of automatically localizing the eosinophilic esophagitis by utilizing spectral information.