Table 1

Antibodies used and immunohistchemical result.


Antibody

Clone

Dilution

Source

Stroma around the micropapillary clusters

Tumor cells

in SMP

outside SMP


Case1

Case2

Case1

Case2

Case1

Case2


MUC1

Ma695

1:100

Novocastra

0

0

2+

2+

1+

2+

EMA

E29

Pre-diluted

Cell Marque

0

0

2+

2+

2+

2+

E-cadherin

NCH38D

1:100

DakoCytomation

0

0

2+

2+

2+

2+

CK

AE1/AE3

Pre-diluted

Nichirei

0

0

2+

2+

2+

2+

TTF-1

8G7G3/1

1:100

DakoCytomation

0

0

1+

2+

2+

2+

SP-A

PE10

1:100

Dako

0

0

1+

0

2+

2+

D2-40

D2-40

Pre-diluted

Nichirei

0

0

0

0

0

0

CD34

Nu-4A1

Pre-diluted

Nichirei

0

0

0

0

0

0

FactorVIII

polyclonal

1:200

DAKO

0

0

0

0

0

0


EMA, epithelial membrane antigen; CK, cytokeratin; TTF-1, thyroid transcription factor-1; SP-A, surfactant protein A.

Novocastra, Newcastle Upon Tyne, UK; Cell Marque, Hot springs, AR, USA; DakoCytomation, Carpinteria, CA, USA; Dako, Kyoto, Japan; Nichirei, Tokyo, Japan; DAKO, Glostrup, Denmark.

0, no positive cells; 1+, positive tumor cells less than 10%; 2+, 10% or more.

Ohe et al. Diagnostic Pathology 2011 6:92   doi:10.1186/1746-1596-6-92

Open Data