Myocardial findings in case 1. (A) Infiltration of polymorphonuclear leukocytes (CD15), without fibrin or hemorrhage, into the dead myocardial tissue. (B) Immunohistochemical analysis for the phenotypic characterization of the cells revealed a positive reaction to the antibody directed against of polymorphonuclear leukocytes (CD15). (C) Confocal laser microscopy: double immunohistochemical reactions for the phenotypic characterization revealed a positive reaction to the antibodies directed against of CD45 (blue reaction) and CD15 (green reaction). (D) Myocyte nuclei labelled by TUNEL assay (apoptosis) revealed an intensive, wide, positive reaction (brown nuclei).
Bello et al. Diagnostic Pathology 2011 6:8 doi:10.1186/1746-1596-6-8