Table 1 |
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List of PCR primers used for EBV subtyping and detection of LMP1 gene deletion |
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Type of Primer |
DNA sequence |
Product size |
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|
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EBNA2 |
EBV type A |
2A-5' |
5'-AACTTCAACCCACACCATCA-3' |
116 bp |
|
EBV type B |
2A-3' |
5'-TTCTGGACTATCTGGATCAT-3' |
120 bp |
|
|
|
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EBV LMP-1 |
wild-type |
1-A |
5'-CGACTCTGCTGGAAATGATGGAGGC-3' |
210 bp |
|
deletion-type |
1-B |
5'-TGAACTGGGCCGTGGGGGTCGTCAT-3' |
180 bp |
|
|
|
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|
β-actin (semi-nested PCR) |
1st forward |
SFW |
5'-CAAGAGATGGCCACGGCTGCT-3' |
|
|
2nd forward |
S4FW |
5'-GAGGCACTCTTCCAGCCTTCC-3' |
148 bp |
|
|
reverse |
A5RV |
5'-TCGTGGATGCCACAGGACTCC-3' |
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|
|
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EBNA2, Epstein-Barr virus nuclear antigen 2 gene; LMP1, latent membrane protein 1 gene |
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Pongpruttipan et al. Diagnostic Pathology 2011 6:79 doi:10.1186/1746-1596-6-79 |
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