Table 1

List of PCR primers used for EBV subtyping and detection of LMP1 gene deletion

Type of Primer

DNA sequence

Product size


EBNA2

EBV type A

2A-5'

5'-AACTTCAACCCACACCATCA-3'

116 bp

EBV type B

2A-3'

5'-TTCTGGACTATCTGGATCAT-3'

120 bp


EBV LMP-1

wild-type

1-A

5'-CGACTCTGCTGGAAATGATGGAGGC-3'

210 bp

deletion-type

1-B

5'-TGAACTGGGCCGTGGGGGTCGTCAT-3'

180 bp


β-actin (semi-nested PCR)

1st forward

SFW

5'-CAAGAGATGGCCACGGCTGCT-3'

2nd forward

S4FW

5'-GAGGCACTCTTCCAGCCTTCC-3'

148 bp

reverse

A5RV

5'-TCGTGGATGCCACAGGACTCC-3'


EBNA2, Epstein-Barr virus nuclear antigen 2 gene; LMP1, latent membrane protein 1 gene

Pongpruttipan et al. Diagnostic Pathology 2011 6:79   doi:10.1186/1746-1596-6-79

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