qRT-PCR Data analysis: Delta Rn vs Cycle (A, B): Real-time PCR amplification plot showing EBER1, EBER2 and IL-10 targets in EBV+ (positive) tumor sample in comparison with EBV Negative control sample analyzed at 0.1 threshold. Successful amplifications are shown, with absence of amplification of mismatched templates. (C) Relative Expression rate of EBER1, EBER2 and IL-10 were calculated by dividing the total threshold (0.10) with mean CT value of each sample.(D) Grid representation of specificity showing mean CT values for on-target cDNA amplification for all EBV+ (positive) specimens. Note the absence of nonspecific amplification. N.D*= Not Detected.
Sheikh and Qadri Diagnostic Pathology 2011 6:70 doi:10.1186/1746-1596-6-70