Table 2 |
|||
|
Quantitative methylation-specific PCR primer and TaqMan probe sequences used in the present study |
|||
|
Genes |
Forward primer sequence (5'→3') |
Probe sequence (5'→3') |
Reverse primer sequence (5'→3') |
|
|
|||
|
CALCA |
GTTTTGGAAGTATGAGGGTGACG |
6FAM-ATTCCGCCAATACACAACAACCAATAAACG-TAMRA |
TTCCCGCCGCTATAAATCG |
|
|
|||
|
CDH1 |
AATTTTAGGTTAGAGGGTTATCGCGT |
6FAM-CGCCCACCCGACCTCGCAT-TAMRA |
TCCCCAAAACGAAACTAACGAC |
|
|
|||
|
DAPK1 |
GGATAGTCGGATCGAGTTAACGTC |
6FAM-TTCGGTAATTCGTAGCGGTAGGGTTTGG-TAMRA |
CCCTCCCAAACGCCGA |
|
|
|||
|
IRX2 |
GCGGGTCGTTTAGGTTAGTATTCG |
6FAM-CCCTCCATCCACGCCCGACCGAAA-TAMRA |
CGCCGAACAACGAACAAAACG |
|
|
|||
|
TIMP3 |
GCGTCGGAGGTTAAGGTTGTT |
6FAM-AACTCGCTCGCCCGCCGAA-TAMRA |
CTCTCCAAAATTACCGTACGCG |
|
|
|||
|
PAX6 |
ATATAGGACGGCGGTTTAGGTTG |
6FAM-CCCAAAATCCGACCGACTCCAACCCCTA-TAMRA |
TTCCGACCGAACGAAAACCTAC |
|
|
|||
|
β-Actin |
TGGTGATGGAGGAGGTTTAGTAAGT |
6FAM-ACCACCACCCAACACACAATAACAAACACA-TAMRA |
AACCAATAAAACCTACTCCTCCCTTAA |
|
|
|||
|
Ji et al. Diagnostic Pathology 2011 6:48 doi:10.1186/1746-1596-6-48 |
|||
