Table 1 |
|||||||
|
Antibodies and immunohistochemical procedures |
|||||||
|
Antibody |
Source |
Type |
Clone |
Positive control |
Dilution |
Incubation temperature |
Incubation time |
|
|
|||||||
|
Ki-67/MIB-1 |
Dako, Glostrup, DK |
Monoclonal |
Ki-67 antigen |
Tonsil |
1:100 |
Room temperature |
40 min |
|
PHH3 |
Upstate Biotechnology. Millipore, Billerica, USA |
Polyclonal |
H3 (Ser10) |
Tonsil |
1:2000 |
Room temperature |
60 min |
|
Survivin |
Abcam Products, Cambridge, MA, USA |
Monoclonal |
EP2880Y |
Tonsil |
1:250 |
Room temperature |
40 min |
|
Mitosin |
Novus Biologicals, LTD, Cambridge, UK |
Monoclonal |
14C10/1D8 |
Tonsil |
1:500 |
Room temperature |
40 min |
|
DNA topoisomerase IIα |
Dako, Glostrup, DK |
Monoclonal |
KiS1 |
Tonsil |
1:100 |
Room temperature |
40 min |
|
|
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|
Habberstad et al. Diagnostic Pathology 2011 6:43 doi:10.1186/1746-1596-6-43 |
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