Table 1

Antibodies and immunohistochemical procedures

Antibody

Source

Type

Clone

Positive control

Dilution

Incubation temperature

Incubation time


Ki-67/MIB-1

Dako, Glostrup, DK

Monoclonal

Ki-67 antigen

Tonsil

1:100

Room temperature

40 min

PHH3

Upstate Biotechnology. Millipore, Billerica, USA

Polyclonal

H3 (Ser10)

Tonsil

1:2000

Room temperature

60 min

Survivin

Abcam Products, Cambridge, MA, USA

Monoclonal

EP2880Y

Tonsil

1:250

Room temperature

40 min

Mitosin

Novus Biologicals, LTD, Cambridge, UK

Monoclonal

14C10/1D8

Tonsil

1:500

Room temperature

40 min

DNA topoisomerase II╬▒

Dako, Glostrup, DK

Monoclonal

KiS1

Tonsil

1:100

Room temperature

40 min


Habberstad et al. Diagnostic Pathology 2011 6:43   doi:10.1186/1746-1596-6-43

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