Table 1

Souce, dilution, pretreatment and cutoff values of primary antibodies used

Antibody (clone)

Manufacturer

Dilution

Pretreatment

Cutoff values


Maspin (EAW24)

Novocastra

1:200

Microwave

≧10% (positive)

CK5/6 (D5/16B4)

Dako

1:50

Water bath

≧10% (positive)

CK14 (LL002)

Novocastra

1:100

Water bath

≧10% (positive)

EGFR (EGFR.113)

Novocastra

1:25

Microwave

≧10% (positive)

CD10 (56C6)

Novocastra

1:50

Water bath

≧10% (positive)

p63 (4A4+Y4A3)

LabVision

1:200

None

≧10% (positive)

ER (1D5)

Dako

1:50

Water bath

≧10% (positive)

PgR (PgR636)

Dako

1:800

Water bath

≧10% (positive)

HER2

Dako

Prediluted (Hercep test)

Water bath

Score 3+ (positive)


Umekita et al. Diagnostic Pathology 2011 6:36   doi:10.1186/1746-1596-6-36

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