Table 1

Standard antibody panel used in the diagnostics of B-cell lymphomas

FITC

PE

PerCP/PerCP-Cy5.5

APC


Test tube

mAbs

Volume

(μl)

mAbs

Volume

(μl)

mAbs

Volume (μl)

mAbs

Volume

(μl)


1**

CD3*

5

CD19*

5

CD45*

5

CD20

3

2**

γ1-FITC

5

γ1-PE

5

CD19

5

γ1-APC

3

3**

Kappa*

5

Lambda*

5

CD19*

5

CD10

3

4**

FMC7*

5

CD23*

5

CD19*

5

CD5

3

5

CD52

3

CD11

5

CD19

5

CD38

3

6

CD4*

5

CD8*

5

CD3*

5

CD2

3


mAbs monoclonal antibodies, FITC fluorescein isothiocyanate, PE phycoerythrin, PerCP peridinin chlorophyll protein, PerCP-Cy5.5 peridinin chlorophyll protein-Cy5.5, APC-allophycocyanin, *BD Oncomark Reagents, ** when the samples were poorly cellular only test tubes 1-4 were applied

Prevodnik et al. Diagnostic Pathology 2011 6:33   doi:10.1186/1746-1596-6-33

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