Table 1 |
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|
Standard antibody panel used in the diagnostics of B-cell lymphomas |
||||||||
|
FITC |
PE |
PerCP/PerCP-Cy5.5 |
APC |
|||||
|
|
||||||||
|
Test tube |
mAbs |
Volume (μl) |
mAbs |
Volume (μl) |
mAbs |
Volume (μl) |
mAbs |
Volume (μl) |
|
|
||||||||
|
1** |
CD3* |
5 |
CD19* |
5 |
CD45* |
5 |
CD20 |
3 |
|
2** |
γ1-FITC |
5 |
γ1-PE |
5 |
CD19 |
5 |
γ1-APC |
3 |
|
3** |
Kappa* |
5 |
Lambda* |
5 |
CD19* |
5 |
CD10 |
3 |
|
4** |
FMC7* |
5 |
CD23* |
5 |
CD19* |
5 |
CD5 |
3 |
|
5 |
CD52 |
3 |
CD11 |
5 |
CD19 |
5 |
CD38 |
3 |
|
6 |
CD4* |
5 |
CD8* |
5 |
CD3* |
5 |
CD2 |
3 |
|
|
||||||||
|
mAbs monoclonal antibodies, FITC fluorescein isothiocyanate, PE phycoerythrin, PerCP peridinin chlorophyll protein, PerCP-Cy5.5 peridinin chlorophyll protein-Cy5.5, APC-allophycocyanin, *BD Oncomark Reagents, ** when the samples were poorly cellular only test tubes 1-4 were applied |
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|
Prevodnik et al. Diagnostic Pathology 2011 6:33 doi:10.1186/1746-1596-6-33 |
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