Table 1

Antibodies used for the immunohistochemical examination

Antibody

Clone

Source

Dilution

Antigen retrieval

Interpretation

CD44 extracellular domain (aminoterminal end)

Polyclonal, Ab64929

Abcam, Cambridge,

MA

1/50

Not applied

Sinicrope's method*

Zo-1

Polyclonal, 61-7300

Zymed, South San Francisco, CA

1/100

Protease treatment (15 min, 37°C)

Linear or dot-like

Occludin

Polyclonal, 71-1500

Zymed

1/100

Protease treatment (15 min, 37°C)

Linear or dot-like

MMP-7

Monoclonal, 141-7B2

Daiichi Fine Chemical, Takaoka, Japan

1/200

Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])

Sinicrope's method*

Pancytokeratin

Monoclonal, MNF116

Dakocytomation, Glostrup, Denmark

1/50

Microwave treatment for 15 min (Dako target retrieval solution [pH 9.0])

For evaluation of tumor budding

beta-Catenin

Monoclonal, 14/beta-catenin

BD Transduction laboratories, Lexington, KY

1/200

Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])

Nuclear, more than 20% judged as positive; membranous and cytoplasmic, Sinicrope's method*

Laminin-5γ2

Monoclonal, 4G1

Dakocytomation

1/50

Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])

Sinicrope's method*

Sialyl Lewis X

Monoclonal, FH6

Ohtsuka Pharmaceutical, Tokushima, Japan

1/2000

Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])

Sinicrope's method*

• Sinicrope's method is described in Reference [27].

Mikami et al. Diagnostic Pathology 2011 6:30   doi:10.1186/1746-1596-6-30