Figure 3.

Representative results for MS-HRM based screening for RASSF1A methylation. Panel 1A, show the sensitivity of the assays with MS-HRM profile characteristic for 100% - blue, 10% - green, 1%- pink and 0% - red mixes of methylated template in unmethylated background. Panel 1B, example of PB sample positive (orange) for methylation. Panel 1C, examples of the tumor sample positive (orange) and negative (brown) for methylation.