Representative results for MS-HRM based screening for BRCA1 methylation. Panel 1A, show the sensitivity of the assays with MS-HRM profile characteristic for 100% - blue, 10% - green, 1%- pink and 0% - red, mixes of methylated template in unmethylated background. Panel 1B, examples of PB sample positive (orange) and negative (brown) for methylation. Panel 1C, examples of the tumor sample positive (orange) and negative (brown) for methylation.
Wojdacz et al. Diagnostic Pathology 2011 6:116 doi:10.1186/1746-1596-6-116