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Open Access Research

Changes of microRNAs-192, 196a and 203 correlate with Barrett's esophagus diagnosis and its progression compared to normal healthy individuals

Pavla Luzna1, Jan Gregar2, Ivo Uberall3, Lenka Radova4, Vlastimil Prochazka2 and Jiri Ehrmann13*

Author affiliations

1 Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Hnevotinska 3, 775 15 Olomouc, Czech Republic

2 Department of Internal Medicine II - Gastroenterology and Hepatology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I.P. Pavlova 6, 779 00 Olomouc, Czech Republic

3 Department of Clinical and Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Hnevotinska 3, 775 15 Olomouc, Czech Republic

4 Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Hnevotinska 3, 775 15 Olomouc, Czech Republic

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Citation and License

Diagnostic Pathology 2011, 6:114  doi:10.1186/1746-1596-6-114

Published: 17 November 2011

Abstract

Background

Barrett's esophagus (BE) is a disease with a rising prevalence in western countries probably due to the unhealthy lifestyle. In significant number of cases it develops to esophageal adenocarcinoma. Two decades ago, important gene regulators (microRNAs) were discovered and their attendance in the process of malignant transformation was demonstrated (e.g. miR-192, 196a, 203). Our aim was to select the patients with the increased risk of malignant transformation before the cancer develops.

Methods

71 patients with BE disease were selected, slides from FFPE blocks were prepared, the lesions were microdissected and a qPCR relative expression analysis for selected microRNAs (generally known to be connected with malignant transformation process) was carried out.

Results

We demonstrated unequivocal statistically significant upregulation of two microRNAs (miR-192, 196a) and downregulation of miR-203 and positive miR-196a correlation with progression from intestinal metaplasia to adenocarcinoma compared to normal individuals.

Conclusions

We hypothetize that there do exist changes of selected microRNAs which can undoubtedly distinguish the patients with BE from normal healthy individuals.

Keywords:
microRNA; Barrett's esophagus; real-time PCR