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Open Access Research

Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma

Zsófia Balogh1, Zsuzsanna Szemlaky1, Miklós Szendrői2, Imre Antal2, Zsuzsanna Pápai3, László Fónyad1, Gergő Papp1, Yi C Changchien1 and Zoltán Sápi1*

Author affiliations

1 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

2 Semmelweis University Orthopedic Clinic, Budapest, Hungary

3 Military Hospital-State Health Centre Department of Oncology, Budapest, Hungary

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Citation and License

Diagnostic Pathology 2011, 6:107  doi:10.1186/1746-1596-6-107

Published: 3 November 2011

Abstract

Background

Although synovial sarcoma is the 3rd most commonly occurring mesenchymal tumor in young adults, usually with a highly aggressive clinical course; remarkable differences can be seen regarding the clinical outcome. According to comparative genomic hybridization (CGH) data published in the literature, the simple and complex karyotypes show a correlation between the prognosis and clinical outcome. In addition, the connection between DNA ploidy and clinical course is controversial. The aim of this study was using a fine-tuning interpretation of our DNA ploidy results and to compare these with metaphase high-resolution CGH (HR-CGH) results.

Methods

DNA ploidy was determined on Feulgen-stained smears in 56 synovial sarcoma cases by image cytometry; follow up was available in 46 cases (average: 78 months). In 9 cases HR-CGH analysis was also available.

Results

10 cases were found DNA-aneuploid, 46 were DNA-diploid by image cytometry. With fine-tuning of the diploid cases according to the 5c exceeding events (single cell aneuploidy), 33 cases were so called "simple-diploid" (without 5c exceeding events) and 13 cases were "complex-diploid"; containing 5c exceeding events (any number). Aneuploid tumors contained large numbers of genetic alterations with the sum gain of at least 2 chromosomes (A-, B- or C-group) detected by HR-CGH. In the "simple-diploid" cases no or few genetic alterations could be detected, whereas the "complex-diploid" samples numerous aberrations (equal or more than 3) could be found.

Conclusions

Our results show a correlation between the DNA-ploidy, a fine-tuned DNA-ploidy and the HR-CGH results. Furthermore, we found significant correlation between the different ploidy groups and the clinical outcome (p < 0.05).

Keywords:
High-Resolution Comparative Genomic Hybridization; HR-CGH; synovial sarcoma; DNA ploidy; clinical outcome; SYT; SSX