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Open Access Research

Expression of MK-1 and RegⅣ and its clinicopathological significances in the benign and malignant lesions of gallbladder

Leping Yang12*, Sigen Lan1, Jieqiong Liu1 and Zhulin Yang1*

Author affiliations

1 Research laboratory of Hepatobiliary Diseases, Second Xiangya Hospital of Central South University, Changsha 410011,China

2 Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA

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Citation and License

Diagnostic Pathology 2011, 6:100  doi:10.1186/1746-1596-6-100

Published: 21 October 2011

Abstract

Background

To study the expression of MK-1 and RegⅣ and to detect their pathological significances in benign and malignant lesions of gallbladder.

Methods

The expression of MK-1 and RegⅣ was detected by immunohistochemical method in paraffin-embedded sections of surgical resected specimens from gallbladder adenocarcinoma (n = 108), peritumoral tissues (n = 46), adenomatous polyp (n = 15), and chronic cholecystitis (n = 35).

Results

The positive rate of MK-1 or RegⅣ expression was significantly higher in gallbladder adenocarcinoma than that in peritumoral tissues (χ2MK-1 = 18.76, P < 0.01; χ2RegⅣ = 9.92, P < 0.01), denomatous polyp (χ2MK-1 = 9.49, P < 0.01; χ2RegⅣ = 8.59, P < 0.01) and chronic cholecystitis (χ2MK-1 = 24.11, P < 0.01; χ2RegⅣ = 19.24, P < 0.01). The positive cases of MK-1 and/or RegⅣ in the benign lesions showed moderately- or severe-atypical hyperplasia of gallbladder epitheli. The positive rates of MK-1 were significantly higher in the cases of well-differentiated adenocarcinoma, no-metastasis of lymph node, and no-invasiveness of regional tissues than those in the ones of differentiated adenocarcinoma, metastasis of lymph node, and invasiveness of regional tissues in gallbladder adenocarcinoma (P < 0.05 or P < 0.01). On the contrary, the positive rates of RegⅣ were significantly lower in the cases of well-differentiated adenocarcinoma, no-metastasis of lymph node, and no-invasiveness of regional tissues than those in the ones of differentiated adenocarcinoma, metastasis of lymph node, and invasiveness of regional tissues in gallbladder adenocarcinoma (P < 0.05 or P < 0.01). Univariate Kaplan-Meier analysis showed that decreased expression of MK-1 (P = 0.09) or increased expression of RegⅣ (P = 0.003) was associated with decreased overall survival. Multivariate Cox regression analysis showed that decreased expression of MK-1 (P = 0.033) and increased expression of RegⅣ (P = 0.008) was an independent prognostic predictor in gallbladder adenocarcinoma.

Conclusions

The expression of MK-1 and/or RegⅣ might be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma.

Keywords:
gallbladder neoplasms; gallbladder polyp; chronic cholecystitis; tumor-associated antigen; MK-1; regenerating gene Ⅳ; immunohistochemistry