Table 1

Weigert-Lead stain (M. Jondet)

Staining solutions

      1 - Weigert's iron haematoxylin

         a - Haematoxylin solution

            Haematoxylin

1 g

            Ethanol

100 ml

         b - Iron solution

            30% Ferric chloride solution in water

4 ml

            Hydrochloric acid 1 M

1 ml

            Distilled water

95 ml

   Mix equal volumes of solution a and b, use within 60 min

      2 - Lead citrate

            lead nitrate

1.33 g

            sodium citrate

2.10 g

            distilled water

30 ml

   Add sodium hydroxyde 0,4 M untill precipitate dissolves

Method

   a - dewax and rehydrate sections

   b - Weighert's haematoxylin

1 minute

   c - wash with distilled water

   d - saturated picric acid in water

1 minute

   e - wash with distilled water

   f - lead citrate

1 minute

   g - wash with water

   h - mount in Eukitt after alcohol and xylene clarification

Results

            Nucleus

Black

            Nuclear membrane

dark black

            Cytoplasm

light grey

Jondet et al. Diagnostic Pathology 2010 5:7   doi:10.1186/1746-1596-5-7