HDGF was highly expressed in HepG2 cells and this level was successfully knocked down by shRNA. A. Total RNA was extracted from HepG2 cells and RT-PCR was applied to examine the steady-state mRNA levels of HDGF and GAPDH. B and C. Parental HepG2 cells and those stably transfected with either control (shCtrl) or HDGF-specific (shHDGF) shRNA were examined for HDGF expression by quantitative real-time PCR and Western-blot. GAPDH and α-tubulin were used as internal control, respectively.
Zhou et al. Diagnostic Pathology 2010 5:58 doi:10.1186/1746-1596-5-58