Table 3

Results of immunohistochemical markers response in neurons

Acute (A) (≤8 hs) Median Expression

Non-acute (NA) (≥8 hs ≤48 hs) Median Expression

Controls (C) Median Expression

Significance Level


GFAP

-

-

A vs C

ns

NA vs C

ns

A vs NA

ns


TNF-α

-

+

-

A vs C

***

NA vs C

ns

A vs NA

***


IL-1β

+

++

-

A vs C

***

NA vs C

***

A vs NA

***


IL-6

-

+

-

A vs C

***

NA vs C

ns

A vs NA

***


MAC387

-

-

-

A vs C

ns

NA vs C

ns

A vs NA

ns


HSP 27

-

-

-

A vs C

ns

NA vs C

ns

A vs NA

ns


HSP 70

+

+++

-

A vs C

***

NA vs C

**

A vs NA

***


HSP 90

+

+++

-

A vs C

***

NA vs C

**

A vs NA

***


COX2

+++

+

-

A vs C

***

NA vs C

***

A vs NA

**


ORP-150

++

+

-

A vs C

***

NA vs C

***

A vs NA

**


β-APP

+

+++

++

A vs C

***

NA vs C

**

A vs NA

**


TrypH

-

+

-

A vs C

***

NA vs C

ns

A vs NA

***


GAP-43

-

-

-

A vs C

ns

NA vs C

ns

A vs NA

ns


Apoptosis (TUNEL)

+

++

-

A vs C

***

NA vs C

**

A vs NA

***


The reactions were graded as follows: 1. (0): not expressed, 2. (+): isolated and disseminated expression, 3. (++): expression in groups or widespread foci, 4. (+++): widespread expression.

NS = p > 0.05, * = p < 0.05, ** = p < 0.01, *** = p < 0.001

Riezzo et al. Diagnostic Pathology 2010 5:49   doi:10.1186/1746-1596-5-49

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