Table 1

Panel of antibodies selected in the study

Antibody against

Pre--treatment

Incubation time of primary antibody, temperature

Concentration of primary antibody


GFAP (Dako, Copenhagen, Denmark)

No pre-treatment

120 min, 20°C

1:300


TNFα (Santa Cruz, CA, USA)

boiling in 0.1 M Citric Acid buffer.

120 min, 20°C

1:600


IL-1β (Santa Cruz, CA, USA)

boiling in 0.25 mM EDTA buffer.

120 min, 20°C

1:4000


IL-6 (Santa Cruz, CA, USA)

5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C.

120 min, 20°C

1:2000


CD68 (Serotec, United Kingdom)

5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C.

120 min, 20°C

1:200


HSP 27 (NovaCastra, United Kingdom)

boiling in 0.25 mM EDTA buffer..

120 min, 20°C

1:20


HSP 70 (NovaCastra, United Kingdom)

boiling in 0.25 mM EDTA buffer..

Overnight, 20°C

1:100


HSP 90 (NovaCastra, United Kingdom)

boiling in 0.25 mM EDTA buffer..

120 min, 20°C

1:50


COX2 (Santa Cruz, CA, USA)

5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C.

120 min, 20°C

1:100


ORP-150 (IBL, Gunma, Japan)

boiling in 0.1 M Citric Acid buffer.

120 min, 20°C

1:200


β-APP (NovaCastra, United Kingdom)

boiling in 0.1 M Citric Acid buffer.

Overnight, 20°C

1:300


TrypH (NovaCastra, United Kingdom)

boiling in 0.1 M Citric Acid buffer.

Overnight, 20°C

1:50


GAP-43 (Santa Cruz, CA, USA)

boiling in 0.1 M Citric Acid buffer

120 min, 20°C

1:2000


TdT enzyme (Chemicon, CA, USA)

15 min in Proteinase K (20 μg/ml), 20°C

60 min, 38°C

30 μl of TdT in 70 μl of reaction buffer (Chemicon, CA, USA)


Riezzo et al. Diagnostic Pathology 2010 5:49   doi:10.1186/1746-1596-5-49

Open Data