Table 1

Immunohistochemical markers used for malignant mesothelioma diagnosis in this study

IHC marker

Antigen type

Clone

Producer

Code No.

Dilution


Positive markers

Calretinin

Calcium-binding protein

polyclonal

Zymed

18-0211

1: 1,500

EMA

HMFG protein

E-29

Dako

M0613

1: 750

Thrombomodulin

endothelial cell transmembrane glycoprotein

1009

Dako

M0617

1: 50

HBME-1

mesothelial cell membrane protein

HBME-1

Dako

M3505

1: 50

Mesothelin

cell surface glycoprotein

5B2

Novocastra

NCL-L-MESO

1: 10

Podoplanin

transmembrane mucoprotein

n.s., monoclonal

Angiobio

11-003

1: 50

CK5/6

IMF

D5/16 B4

Dako

M7237

1: 80

CK7

IMF

OV-TL12/30

Dako

M7018

1: 1,000

CK AE1/AE3

IMF (pan-CK cocktail, subfamilies A and B)

AE1 and AE3

Dako

N1590

1: 75

CK KL1

IMF (pan-CK cocktail)

KL1

Serotec

MCA144H

1: 50

Negative markers

monoclonal CEA

Gold 1 epitope

II-7

Dako

M7072

1: 200

polyclonal CEA

CEA and CEA-like proteins

polyclonal

Dako

A0115

1: 13,000

Ber-Ep4

transmembrane glycoprotein

Ber-Ep4

Dako

M 0804

1: 200

CD15

Lewis X carbohydrate antigen

C3D-1

Dako

M0733

1: 25

Sialyl-TN

Sialosyl-Tn1 glycoprotein

HB-STn1

Dako

M0899

1: 100

TTF-1

nuclear transcription factor

8G7G3/1

Dako

M3575

1: 100

Others

CK20

IMF

Ks 20.8

Dako

M7019

1: 200

CD10

neutral endopeptidase 24.11

SS2/36

Dako

M0727

1: 50

CD34

single-chain transmembrane protein

QBEnd 10

Dako

M7165

1: 100

CD68

glycosylated lysosomal membrane protein

KP1

Dako

M814

1: 3,000

CD99

MIC2 gene products

12E7

Dako

M3601

1: 100

Bcl-2

apoptosis inhibitor

124

Dako

M0887

1: 200

SMA

microfilaments

1A4

Dako

M0851

1: 300

Desmin

IMF

D33

Dako

M0760

1: 100

S-100

Calcium-binding proteins

polyclonal

Dako

Z0311

1: 3,000

HMB-45

part of neuraminidase-sensitive glycoconjugate in melanosomes

HMB45

Dako

M0634

1: 100

HHF-35

muscle actin

HHF 35

Dako

M0635

1: 200

MIB1

nuclear protein, proliferation marker

Ki-67/MIB-1

Dako

M7240

1: 200


Abbreviations:

IMF - intermediate filament; EMA - Epithelial Membrane Antigen; HMFG - Human Milk Fat Globule; HBME - Hector Battifora Mesothelial (Human Mesothelial Cell Antigen); CK - Cytokeratin; AE - Anti-Epithelial; KL1 - Keratin L1; CD - Cluster of Differentiation; TTF - Thyroid Transcription Factor; Bcl - B-cell lymphoma; SMA - Smooth Muscle Actin; HMB - Human Melanoma Black; HHF - Human Heart Fibroblasts/myofibroblasts; MIB - Molecular Immunology Borstel; Ki - Kiel

Sandeck et al. Diagnostic Pathology 2010 5:47   doi:10.1186/1746-1596-5-47

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