Table 1 |
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|
Immunohistochemical markers used for malignant mesothelioma diagnosis in this study |
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|
IHC marker |
Antigen type |
Clone |
Producer |
Code No. |
Dilution |
|
|
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|
Positive markers |
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|
Calretinin |
Calcium-binding protein |
polyclonal |
Zymed |
18-0211 |
1: 1,500 |
|
EMA |
HMFG protein |
E-29 |
Dako |
M0613 |
1: 750 |
|
Thrombomodulin |
endothelial cell transmembrane glycoprotein |
1009 |
Dako |
M0617 |
1: 50 |
|
HBME-1 |
mesothelial cell membrane protein |
HBME-1 |
Dako |
M3505 |
1: 50 |
|
Mesothelin |
cell surface glycoprotein |
5B2 |
Novocastra |
NCL-L-MESO |
1: 10 |
|
Podoplanin |
transmembrane mucoprotein |
n.s., monoclonal |
Angiobio |
11-003 |
1: 50 |
|
CK5/6 |
IMF |
D5/16 B4 |
Dako |
M7237 |
1: 80 |
|
CK7 |
IMF |
OV-TL12/30 |
Dako |
M7018 |
1: 1,000 |
|
CK AE1/AE3 |
IMF (pan-CK cocktail, subfamilies A and B) |
AE1 and AE3 |
Dako |
N1590 |
1: 75 |
|
CK KL1 |
IMF (pan-CK cocktail) |
KL1 |
Serotec |
MCA144H |
1: 50 |
|
Negative markers |
|||||
|
monoclonal CEA |
Gold 1 epitope |
II-7 |
Dako |
M7072 |
1: 200 |
|
polyclonal CEA |
CEA and CEA-like proteins |
polyclonal |
Dako |
A0115 |
1: 13,000 |
|
Ber-Ep4 |
transmembrane glycoprotein |
Ber-Ep4 |
Dako |
M 0804 |
1: 200 |
|
CD15 |
Lewis X carbohydrate antigen |
C3D-1 |
Dako |
M0733 |
1: 25 |
|
Sialyl-TN |
Sialosyl-Tn1 glycoprotein |
HB-STn1 |
Dako |
M0899 |
1: 100 |
|
TTF-1 |
nuclear transcription factor |
8G7G3/1 |
Dako |
M3575 |
1: 100 |
|
Others |
|||||
|
CK20 |
IMF |
Ks 20.8 |
Dako |
M7019 |
1: 200 |
|
CD10 |
neutral endopeptidase 24.11 |
SS2/36 |
Dako |
M0727 |
1: 50 |
|
CD34 |
single-chain transmembrane protein |
QBEnd 10 |
Dako |
M7165 |
1: 100 |
|
CD68 |
glycosylated lysosomal membrane protein |
KP1 |
Dako |
M814 |
1: 3,000 |
|
CD99 |
MIC2 gene products |
12E7 |
Dako |
M3601 |
1: 100 |
|
Bcl-2 |
apoptosis inhibitor |
124 |
Dako |
M0887 |
1: 200 |
|
SMA |
microfilaments |
1A4 |
Dako |
M0851 |
1: 300 |
|
Desmin |
IMF |
D33 |
Dako |
M0760 |
1: 100 |
|
S-100 |
Calcium-binding proteins |
polyclonal |
Dako |
Z0311 |
1: 3,000 |
|
HMB-45 |
part of neuraminidase-sensitive glycoconjugate in melanosomes |
HMB45 |
Dako |
M0634 |
1: 100 |
|
HHF-35 |
muscle actin |
HHF 35 |
Dako |
M0635 |
1: 200 |
|
MIB1 |
nuclear protein, proliferation marker |
Ki-67/MIB-1 |
Dako |
M7240 |
1: 200 |
|
|
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|
Abbreviations: IMF - intermediate filament; EMA - Epithelial Membrane Antigen; HMFG - Human Milk Fat Globule; HBME - Hector Battifora Mesothelial (Human Mesothelial Cell Antigen); CK - Cytokeratin; AE - Anti-Epithelial; KL1 - Keratin L1; CD - Cluster of Differentiation; TTF - Thyroid Transcription Factor; Bcl - B-cell lymphoma; SMA - Smooth Muscle Actin; HMB - Human Melanoma Black; HHF - Human Heart Fibroblasts/myofibroblasts; MIB - Molecular Immunology Borstel; Ki - Kiel |
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|
Sandeck et al. Diagnostic Pathology 2010 5:47 doi:10.1186/1746-1596-5-47 |
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