Open Access Research

Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines

Cristian M Ortiz1*, Tetsuo Ito2, Yosuke Hashimoto3, Satoshi Nagayama4, Akira Iwai4, Shigeru Tsunoda4, Fumiaki Sato5, Miguel Martorell1, Jose Angel Garcia1, Ana Perez1 and Yutaka Shimada6

Author Affiliations

1 Department of Pathology, Valencia University, Hospital General Universitario de Valencia, Avenida tres cruces N° 2, CP 46014, Valencia, Spain

2 Department of of Surgery, Sugita Genpaku Memorial Obama Municipal Hospital. 2-2 Ohte-cho, Obama, Fukui, 917-8567, Japan

3 Division of Colon and Rectal Surgery, Shizuoka Cancer Center Hospital, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777, Japan

4 Department of Surgery, Graduate School of Medicine, Kyoto University, Kawaracho 54 Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

5 Department of Nanobio Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Kawaracho 54 Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

6 Department of Surgery & Science. Graduate School of Medicine & Pharmaceutical Sciences for Research, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan

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Diagnostic Pathology 2010, 5:41  doi:10.1186/1746-1596-5-41

Published: 21 June 2010

Additional files

Additional file 1:

Immunohistochemical staining for β-catenin. Photomicrographs of immunohistochemical staining detected β-catenin active pattern in the in vivo model tumor using stable transfectant cells with an empty vector (KYSE 170 mock) and with a siRNA vector against fascin (KYSE 170F4).

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Additional file 2:

Immunofluorescent staining for β-catenin. Immunofluorescent staining in vitro. KYSE 170 mock cells expressing β-catenin in the cytoplasm and nucleus (middle); DAPI (right); Actin. (left). KYSE 170 F4 cells expressing β-catenin in the cytoplasm and membrane (middle); DAPI (right); Actin. (left). Positive control HCT15 cells expressing strongly β-catenin in the nucleus (middle); DAPI (right); Actin. (left).

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