Table 2

KRAS Mutations Reported by Mutation Analysis Method

Sample

Direct Sequencing (Amgen)

Allele-Specific PCR (HistoGeneX/DxS)

Direct Sequencing (Gentris)

Allele-Specific Hybridization (Invitek)

Allele-Specific PCR Extensions (Genzyme)

Picotiter Plate Sequencing (Agencourt)*


Samples for which a KRAS sequence determination was made, n

40

40

32

27

35

32

Total WT, n

20

21

18

3

15

15

1

G13D

G13D

G13D

G13D

G13D

G13D (4219/7836; 0.54)**

2

WT

WT

WT

Inconclusive

WT

WT (8880)**

3

G13D

G13D

12WT, no call 13

G13D

G13D

G13D (1679/8282; 0.2)

4

WT

WT

WT

Inconclusive

WT

WT (8371)

5

WT

WT

WT

G13D

WT

Not tested

6

G12A

G12A

G12A

WT

G12A

No result

7

G12S, G13D

G12S

G12S, no call 13

Inconclusive

Failed

G12S (53/825; 0.06)

8

G12V, G13D

G12V

G12V

G12S, G12V

G12V

G12V (244/1289; 0.19), G13D (53/1289; 0.04)

9

WT

WT

WT

Not analyzed

Failed

G12V (43/106; 0.41)

10

WT

WT

12WT, no call 13

WT

Failed

WT (18)

11

WT

WT

Mixed sequence§

Not analyzed

Failed

No result

12

G12V

G12V

G12V

G12D, G13D

G12V

G12V (2443/4707; 0.52)

13

WT

WT

WT

G13D

WT

WT (9223)

14

WT

WT

WT

Inconclusive

WT

WT (9921)

15

G12D

G12D

G12D

G12D, G13D

G12D

G12D (4178/8006; 0.52)

16

WT

WT

WT

Inconclusive

WT

WT (7001)

17

G13D

G13D

No call 12 or 13

G13D

G13D

Not tested||

18

G12A

G12A

G12A

G12A, G13D

G12A

Not tested||

19

WT

WT

WT

G13D

WT

WT (8891)

20

G12D

G12D

G12D

G12D

G12D

G12D (2513/4304; 0.58)

21

G13D

G12D

WT

G12D, G13D

G12D, G13D

G12D (761/6260; 0.12), G13D (1639/6260; 0.26)

22

WT

WT

WT

Inconclusive

WT

WT (2908)

23

G12D

G12D

WT

Inconclusive

G12D

G12D (2115/7262; 0.29)

24

WT

WT

No amplification

WT

Failed

WT (71)

25

WT

WT

WT

G13D

WT

WT (2477)

26

WT

WT

WT

Inconclusive

WT

WT (5887)

27

G12V

G12V

G12V

G12V

G12V

G12V (1812/6404; 0.28)

28

WT

WT

WT

Inconclusive

WT

WT (8696)

29

WT

WT

G12V

G13D

WT

WT (3876)

30

G13D

G13D

G13D

G13D

G13D

G13D (4697/9754; 0.48)

31

WT

WT

No amplification

G12D, G13D

G13D

No result

32

WT

WT

WT

G13D

WT

WT (5048)

33

G12V

G12V

WT

G12V, G13D

G12V

G12V (505/1042; 0.48)

34

WT

WT

WT

G13D

WT

WT (6329)

35

G13D

G13D

G13D

G13D

G13D

G13D (2578/7158; 0.36)

36

G12V, G13D

G12V

G12V

Inconclusive

G12V

Not tested||

37

G12D

G12D

G12D

G12D, G13D

G12D

G12D (14/61; 0.23)

38

G13D

WT

12WT, no call 13

G13D

G13D

G13D (1539/5423; 0.28)

39

WT

WT

WT

G13D

WT

Not tested||

40

G12D

G12D

G12D

Inconclusive

G12D

G12D (1884/4565; 0.41)


PCR = polymerase chain reaction; WT = wild type.

*Numbers in parentheses indicate the proportion of sample DNA containing the mutant allele.

Service was unable to conclusively determine genotype.

Sample was determined to be unsuitable for subsequent hybridization.

§Multiple observed sequences precluded deconvolution of sequence traces.

||Samples no longer available for testing.

KRAS sequence could not be amplified by PCR.

**The single number in parentheses refers to the total sequence reads for that sample when all were WT, and for MT, the fraction reflects the number of sequence reads for a given mutation over the total number of sequence reads for that sample, followed by the numerical fraction of that ratio.

Oliner et al. Diagnostic Pathology 2010 5:23   doi:10.1186/1746-1596-5-23

Open Data