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Open Access Research

Expression and prognostic significance of cox-2 and p-53 in hodgkin lymphomas: a retrospective study

Nagehan O Barisik1*, Suheyla Bozkurt2, Mahmut Gumus3, Isik Kaygusuz4, Nimet Karadayi1, Emine Bas2, Mahmut Bayik4 and Tulay Tecimer2

Author Affiliations

1 Kartal Research And Education Hospital Pathology Department/Istanbul-Turkey

2 Marmara University Pathology Department/Istanbul-Turkey

3 Kartal Research And Education Hospital Oncology Department/Istanbul-Turkey

4 Marmara University Hematology Department/Istanbul-Turkey

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Diagnostic Pathology 2010, 5:19  doi:10.1186/1746-1596-5-19

Published: 26 March 2010

Abstract

Background

Cyclooxygenase (cox) is the rate-limiting enzyme, which catalyzes the conversion of arachidonic acid into prostaglandins and contributes to the inflammatory process. Cyclooxygenase-2 (cox-2), which is one of the two isoforms, plays a role in tumor progression and carcinogenesis. p53 contributes to apoptosis, DNA renewal and cell cycle. Studies concerning the relationship of cox-2 and p53 expressions and carcinogenesis are available, but the association between cox-2 and p53 in Hodgkin lymphoma (HL) is not exactly known.

In our study, we examined the association of cox-2 and p53 expression, with age, stage, histopathological subtype, and survival in HL. We also examined correlation between cox-2 and p53 expression.

Methods

Cox-2 and p53 expressions in Hodgkin-Reed Sternberg cells (HRS) were examined in 54 patients with HL depending on cox-2 expression, stained cases were classified as positive, and unstained cases as negative. Nuclear staining of HRS cells with p53 was evaluated as positive. The classifications of positivity were as follows: negative if<10%; (1+) if 10-25%; (2+) if 25-50%; (3+) if 50-75%, (4+) if >75%.

Results

Cox-2 and p53 expressions were found in 49 (80%) and 29 (46%) patients, respectively. There were differences between histological subtypes according to cox-2 expression (p = 0.012). Mixed cellular (MC) and nodular sclerosing (NS) subtypes were seen most of the patients and cox-2 expression was evaluated mostly in the mixed cellular subtype.

There were no statistically significant relationships between p53 and the histopathological subtypes; or between p53, cox-2 and the factors including stage, age and survival; or between p53 and cox-2 expression (p > 0.05).

Conclusion

Considering the significant relationship between the cox-2 expression and the subtypes of HL, cox-2 expression is higher in MC and NS subtypes. However the difference between these two subtypes was not significant. This submission must be advocated by studies with large series