Table 1

erbB antibodies used

Antibody

Source

Type

Clone

Reactant

Positive control

Dilution

Incubation time and temperature


NCL-EGFR-384

Novocastra, Newcastle-upon-Tyne, UK

Monoclonal

EGFR.25

EGFR (c-erbB1 protein) (internal domain)

Human skin

1:100

60 min. at room temperature

NCL-EGFR

Novocastra

Monoclonal

EGFR.113

EGFR (c-erbB1 protein) (external domain)

Human skin

1:10

60 min. at room temperature

c-erbB-2

Novocastra

Monoclonal

CB11

c-erbB2 protein (internal domain)

SKBR-3 breast cancer cell line

1:40

60 min. at room temperature

c-erbB-2

Immunotech, Marseille, France

Monoclonal

3B5

c-erbB2 protein (internal domain)

SKBR-3 breast cancer cell line

Ready to use

60 min. at room temperature

c-erbB-2

Novocastra

Monoclonal

5A2

c-erbB2 protein (internal domain)

SKBR-3 breast cancer cell line

1:50

60 min. at room temperature

NCL-c-erbB-3

Novocastra

Monoclonal

RTJ1

c-erbB-3 protein

Normal kidney

1:10

Over night at 4°C

c-erbB-4/HER4

NeoMarkers (Fremont, CA, USA)

Monoclonal

HFR-1

c-erbB-4 protein

Breast carcinoma

1:10

Over night at 4°C


Gulati et al. Diagnostic Pathology 2010 5:18   doi:10.1186/1746-1596-5-18

Open Data