Table 2 |
|||
|
Primer details: Sequence, annealing temperature (AT), and amplicon size (AS) of target sequence |
|||
|
Target gene |
Sequence (FP; 5' to 3') |
AT [°C] |
AS [bp] |
|
|
|||
|
Sequence (RP; 5' to 3') |
|||
|
|
|||
|
GAPDH |
AATGGTAAGGTCGGTGTGTGAA |
60 |
262 |
|
|
|||
|
GAAGATGGTGATGGGCTTCC |
|||
|
|
|||
|
GAPDH-3' UTR |
ACAGGGTGGTGGACCTCATG |
60 |
102 |
|
|
|||
|
GTTGGGATAGGGCCTCTCTTG |
|||
|
|
|||
|
PBGD |
TGCACGATCCTGAAACTCTG |
60 |
163 |
|
|
|||
|
TGCATGCTATCTGAGCCATC |
|||
|
|
|||
|
28S |
TCATCAGACCCCAGAAAAGG |
60 |
102 |
|
|
|||
|
GATTCGGCAGGTGAGTTGTT |
|||
|
|
|||
|
18S |
GCAATTATTCCCCATGAACG |
60 |
123 |
|
GGCCTCACTAAACCATCCAA |
|||
|
|
|||
|
Muyal et al. Diagnostic Pathology 2009 4:9 doi:10.1186/1746-1596-4-9 |
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