Assessment of RNA fragment sizes. An amplification factor of GAPDH-3'UTR, GAPDH, and PBGD was determined to assess the fragment sizes of RNA. The short (200 bp) and medium (700 bp) sequences were amplified by both techniques with similar fashion in RNA isolated from frozen and fresh lung tissue. More copy numbers of medium fragment (GAPDH) were obtained with fresh compared to frozen using SGC method. RNA isolated from SGC effectively amplified a longer fragment (<14,00 bp) in fresh and frozen lung tissue. In contrast, with the GTC technique a higher copy number of the longer product was obtained with fresh lung tissues only.
Muyal et al. Diagnostic Pathology 2009 4:9 doi:10.1186/1746-1596-4-9