Assessment of total RNA quality. An amplification factor of 28S and 18S subunits of rRNA was determined; a higher copy numbers of 28S (A) was obtained with SGC method in frozen and fresh lung tissue compared with the GTC method. No significant difference was observed for 18S (B) in RNA isolated from frozen lung, however, more copy numbers of 18S was obtained from fresh lung tissues.
Muyal et al. Diagnostic Pathology 2009 4:9 doi:10.1186/1746-1596-4-9