Example of an active sampling to determine the discrimination threshold of a Mib-1 PAP stained undifferentiated large cell carcinoma (in association to the distance of the nearest vessel (proliferating nuclei are stained brown, vessels are stained red). The percentages of stained nuclei in relation to three different thresholds (level 1 – 3) are shown for six different sample areas. The discrimination threshold can be chosen between level 1 and level 2 without major error in contrast to level 3, which would result in wrong (too low) estimates.
Kayser et al. Diagnostic Pathology 2009 4:6 doi:10.1186/1746-1596-4-6