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Electron tomography of negatively stained complex viruses: application in their diagnosis

Jan Mast* and Lien Demeestere

Author Affiliations

EM-unit, CODA-CERVA, Groeselenberg 99, Brussels, Belgium

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Diagnostic Pathology 2009, 4:5  doi:10.1186/1746-1596-4-5

Published: 10 February 2009

Additional files

Additional file 1:

Tomogram of a parapoxvirus. The video shows a negatively stained virion of the Orf parapoxvirus, a section of which is shown in Figure 1C. Each frame of the movie corresponds to a 0.44 nm-thick digital section through the tomogram moving along the tomographic Z-axis (Total Depth nm). Observe the semi-parallel orientation of the surface threads.

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Open Data

Additional file 2:

Surface rendered view of a parapoxvirus. The video shows a surface rendered view of the parapoxvirus particle seen in additional file 1. Figure 1C represents a section of this movie. Observe the surface threads that, instead of being randomly arranged, form a more or less regular spiral

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Open Data

Additional file 3:

False-colored 3D-rendering of a C-particle of a parapoxvirus. This figure shows snapshots of a false-colored 3D-rendering of the C-particles shown along the tomographic Y-axis (A) and Z-axis (B) se-colored rendering surface rendered view of the parapoxvirus particle seen in additional file 1. Figure 1C represents a section of this movie.

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Open Data

Additional file 4:

Tomogram of Newcastle disease virus. The video shows a negatively stained virion of the Newcastle disease LaSota strain, a section of which is shown in Figure 2B. Each frame of the movie corresponds to a 0.44 nm-thick digital section through the tomogram moving along the tomographic Z-axis.

Format: AVI Size: 15.3MB Download file

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Open Data