Table 1

List of Antibodies for Immunohistochemical Studies

Antibody

Clone

Dilution

Antigen retrieval method

Source


Vimentin

V9

1:150

Citrate buffer (20 min)

Neomarkers, Fremont, CA, USA


Epithelial membrane antigen (EMA)

GP1.1

1:150

Citrate buffer (20 min)

DBS, Pleasanton, CA


Pan-cytokeratin

AE1/AE3

1:50

Citrate buffer (20 min)

Neomarkers


Cytokeratin 7

OV-TL 12/30

1:200

Tripsin (10 min at 37°C)

Neomarkers


Cytokeratin 20

Ks20.8

1:100

Tripsin (10 min at 37°C)

Neomarkers


Desmin

D33

1:100

Citrate buffer (20 min)

Neomarkers


α-Actin (SMA)

1A4

1:200

Citrate buffer (20 min)

Neomarkers


S-100 protein

4C4.9

1:150

Citrate buffer (20 min)

Neomarkers


Leukocyte common antigen (LCA)

Cocktail PanLCA

1:200

Citrate buffer (20 min)

Neomarkers


CD68

PG-M1

1:100

Citrate buffer (20 min)

Neomarkers


Ki-67

SP6

1:200

Citrate buffer (20 min)

Neomarkers


P53

DO7

Prediluted

Citrate buffer (20 min)

Dako, Carpenteria, CA, USA


TRAP

26E5

Prediluted

Citrate buffer (20 min)

Neomarkers


Behzatoğlu et al. Diagnostic Pathology 2009 4:48   doi:10.1186/1746-1596-4-48

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