Table 1

List of Antibodies for Immunohistochemical Studies

Antibody

Clone

Dilution

Antigen retrieval method

Source

Vimentin

V9

1:150

Citrate buffer (20 min)

Neomarkers, Fremont, CA, USA

Epithelial membrane antigen (EMA)

GP1.1

1:150

Citrate buffer (20 min)

DBS, Pleasanton, CA

Pan-cytokeratin

AE1/AE3

1:50

Citrate buffer (20 min)

Neomarkers

Cytokeratin 7

OV-TL 12/30

1:200

Tripsin (10 min at 37°C)

Neomarkers

Cytokeratin 20

Ks20.8

1:100

Tripsin (10 min at 37°C)

Neomarkers

Desmin

D33

1:100

Citrate buffer (20 min)

Neomarkers

α-Actin (SMA)

1A4

1:200

Citrate buffer (20 min)

Neomarkers

S-100 protein

4C4.9

1:150

Citrate buffer (20 min)

Neomarkers

Leukocyte common antigen (LCA)

Cocktail PanLCA

1:200

Citrate buffer (20 min)

Neomarkers

CD68

PG-M1

1:100

Citrate buffer (20 min)

Neomarkers

Ki-67

SP6

1:200

Citrate buffer (20 min)

Neomarkers

P53

DO7

Prediluted

Citrate buffer (20 min)

Dako, Carpenteria, CA, USA

TRAP

26E5

Prediluted

Citrate buffer (20 min)

Neomarkers

Behzatoğlu et al. Diagnostic Pathology 2009 4:48   doi:10.1186/1746-1596-4-48