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Open Access Highly Accessed Research

Altered PTEN expression; a diagnostic marker for differentiating normal, hyperplastic and neoplastic endometrium

Soheila Sarmadi1*, Narges Izadi-Mood1, Kambiz Sotoudeh2 and Seyed Mohammad Tavangar3

Author Affiliations

1 Department of Pathology, Mirza Koochak Khan Hospital, Tehran University of Medical Sciences, Tehran, Iran

2 Resident of Pathology, Iran University of Medical Sciences, Tehran, Iran

3 Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

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Diagnostic Pathology 2009, 4:41  doi:10.1186/1746-1596-4-41

Published: 25 November 2009

Abstract

Background

Different molecular alterations have been described in endometrioid endometrial carcinoma (EECA). Among them the most frequently altered is loss of the PTEN protein, a tumor suppressor gene. The purpose of this study was to evaluate the expression pattern of PTEN gene in normal, hyperplastic and neoplastic endometrium.

Methods

In a study in a referral gynecologic hospital in Tehran, Iran, immunohistochemical (IHC) evaluation of PTEN was performed on 87 consecutive specimens to the following three groups; group A- normal proliferative endometrium(n = 29); group B- hyperplastic endometrium [including simple hyperplasia without atypia(n = 21) and complex hyperplasia with atypia (n = 8)] and group C- EECA(n = 29). Immunostaining of cells was analyzed by arbitrary quantitative methods according to both slide's area staining and intensity of color reaction.

Results

PTEN immunoreactivity was present in all normal proliferative endometrium, all simple hyperplasia, 75% of atypical complex hyperplasia and in 48% of EECA (P < 0.001). The intensity of PTEN reaction was significantly higher in group with proliferative endometrium than hyperplastic endometrium and EECA (P < 0.001).

Conclusion

PTEN expression was significantly higher in cyclical endometrium than in atypical hyperplasia and endometrioid carcinoma.