Figure 4.

Enhancement of TLR and NOD ligand-induced production of IL-8 and MCP-1 in human PBMCs preincubated with purified IgGs from PR3-ANCA sera. PBMCs (donor 1 and 2, 2 × 10⁵ cells per ml) were preincubated for 6 h with PR3-ANCA IgG (50 μg/ml, Q9-40 or R9-10) (closed bar) or with an equal amount of normal IgG (sham incubation, open bar). Subsequently, PBMCs were challenged with FSL-1 (1 nM), poly I:C (1 μg/ml), lipid A (10 ng/ml), ssPoly U (10 μg/ml), FK156 (100 μg/ml) or MDP (100 μg/ml) for 18 h. The levels of IL-8 and MCP-1 in the culture supernatants were determined by ELISA, and expressed as means ± S.D. *^,# Significantly different from sham-incubated PBMCs and from respective cultures stimulated with the respective ligands alone. The results are representative of four different experiments.