Enhancement of TLR and NOD ligand-induced IL-8 production in human monocytic THP-1 cells preincubated with PR3-ANCA sera. THP-1 cells (2 × 105 per ml) were preincubated for 6 h with 1:100 dilutions of PR3-ANCA sera from WG patients (Q9-40, R9-10, S9-31, R9-7, S9-29, S9-32, and D10-41) (closed bar) or with equal amounts of normal serum (sham incubation, open bar). Subsequently, THP-1 cells were challenged with FSL-1 (1 nM), poly I:C (1 μg/ml), lipid A (10 ng/ml), ssPoly U (10 μg/ml), CpG DNA (1 μM), FK156 (100 μg/ml), or MDP (100 μg/ml) for 18 h. IL-8 levels in the culture supernatants were determined by ELISA, and expressed as means ± S.D. *,# Significantly different from sham-incubated THP-1 cells and from respective cultures stimulated with the respective ligands alone. The results are representative of three different experiments.
Uehara et al. Diagnostic Pathology 2009 4:23 doi:10.1186/1746-1596-4-23